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Alpha-Tubulin acetyl-K40 kit HTRF®

The Alpha-Tubulin acetyl-K40 kit is designed for the rapid detection of K40 tubulin acetylation in cell lysates.
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  • No-wash No-wash
  • Low sample consumption Low sample consumption
  • High sensitivity High sensitivity
The Alpha-Tubulin acetyl-K40 kit is designed for the rapid detection of K40 tubulin acetylation in cell lysates.
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Overview

Alpha-Tubulin is a member of the tubulin protein superfamily, which polymerizes with the beta tubulin to form microtubules, a major component of the eukaryotic cytoskeleton. Microtubules contribute to several cellular processes, such as structural support, intracellular transport, and DNA segregation. Alpha-Tubulin K40 acetylation has been shown to affect tubulin self-assembly and protect microtubules against stress-induced material fatigue. The Alpha-Tubulin acetyl-K40 kit is designed for the quantitative detection of K40 acetylated Alpha-Tubulin in cell lysates.

Benefits

  • HDAC6 MODULATION STUDY

Assay principle

Alpha-Tubulin acetyl-K40 is measured using a sandwich immunoassay involving two specific anti-alpha-Tubulin antibodies, respectively labelled with Europium Cryptate (donor) and d2 (acceptor). The intensity of the signal is proportional to the concentration of K40 acetylated alpha-Tubulin present in the sample.
Alpha tubullin kit principle

Assay Protocol

The protocol for the alpha-Tubulin acetyl-K40 assay is described here. Cells are plated, stimulated, and lysed in the same 96-well culture plate. Lysates are then transferred to the assay plate for the detection of Alpha-Tubulin by HTRF reagents. This protocol enables the cells' viability and confluence to be monitored. The antibodies labelled with HTRF fluorophores may be pre-mixed and added in a single dispensing step to further streamline the assay procedure. The assay detection can be run in 96- to 384-well plates by simply resizing each addition volume proportionally.
Alpha tubulin kit protocol

Plate Reader Requirement

Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.

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