Human Apolipoprotein E kit
ApoE quantification in cells, supernatants and plasma
This HTRF kit is designed for an accurate quantitative measurement of alpha synuclein aggregation in brain extracts.
Monitoring alpha-synuclein aggregation is of great interest for studying the pathogenesis of synucleinopathies, a group of neurodegenerative diseases that includes Parkinson's disease (PD), dementia with Lewy bodies (DLB), diffuse Lewy body disease (DLBD), and multiple system atrophy (MSA). The kit provides a solution to rapidly detect and quantify protein aggregates of human alpha-synuclein in cell culture or brain tissue extracts.
Samples from human putamen (Parkinson's versus control) were collected and lysed with a Cisbio lysis buffer. Due to the nature of the samples, the conventional cellular protocol needed to be adjusted. Incubation was performed for 20 hours at RT. Even at high dilution (1/64), clear discrimination between the two sample types is possible with Cisbio's aggregated alpha synuclein assay.
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