Cortisol kit
Cortisol quantification from liver microsomes, serum and cells
Aldosterone kit HTRF®
The aldosterone kit is designed for the accurate quantitative measurement of the Aldosterone steroid hormone released by cells.
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No-wash
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Results within 1 hour
Overview
The Aldosterone assay kit accurately measures the release of the Aldosterone steroid hormone in cell supernatant and serum samples. Aldosterone is a steroid hormone synthesized from cholesterol in the adrenal cortex. Aldosterone is metabolized in the kidney and liver, and functions as the key mineralocorticoid in the control of sodium and potassium balance. The Aldosterone kit offers superior benefits over traditional ELISA readouts with the ease of an Add & Read process coupled with the robustness and sensitivity of homogeneous TR-FRET assays.
Benefits
- FULLY QUANTITATIVE
- CELL AND SERUM ASSESSMENT
- ALDOSTERONE SYNTHASE SCREENING
Assay principle
The kit is based on a competitive format involving a specific antibody labelled with Cryptate (donor) and Aldosterone coupled to XL665 (acceptor). Aldosterone produced by cells competes with XL665-labelled Aldosterone for binding to monoclonal anti-Aldosterone Eu Cryptate.
Assay protocol
The Aldosterone assay features a streamlined protocol with only two incubation steps: - Cell stimulation by target compounds - Aldosterone detection using HTRF reagents
This protocol requires only a single, one-hour incubation period following cell stimulation.
Specifications
| Detection limit: | 24 pg/mL |
|---|---|
| Dynamic range: | 0.024 to 160 ng/mL |
| EC50: | 453 pg/mL |
| Specificity: | low cross-reactivity with pregnenolone 0.0003%, progesterone 0.0017%, deoxycorticosterone 0.0017%, corticosterone 0.05% |
Product Insert Aldosterone Kit / 64ALDPEG-64ALDPEH
64ALDPEG-64ALDPEH - Product Insert
All your HTRF assays in one document! - Catalog
A guide to Homogeneous Time Resolved Fluorescence
General principles of HTRF - Guides
How HTRF compares to Western Blot and ELISA
Get the brochure about technology comparison. - Brochures
Safety Data Sheet (DEU) Aldosterone Kit / 64ALDPEG
64ALDPEG - Safety Data Sheet
Safety Data Sheet (ELL) Aldosterone Kit / 64ALDPEG
64ALDPEG - Safety Data Sheet
Safety Data Sheet (FRA-FR) Aldosterone Kit / 64ALDPEG
64ALDPEG - Safety Data Sheet
Safety Data Sheet (ITA) Aldosterone Kit / 64ALDPEG
64ALDPEG - Safety Data Sheet
Safety Data Sheet (SPA) Aldosterone Kit / 64ALDPEG
64ALDPEG - Safety Data Sheet
Safety Data Sheet (ENG-GB) Aldosterone Kit / 64ALDPEG
64ALDPEG - Safety Data Sheet
Safety Data Sheet (ENG-US) Aldosterone Kit / 64ALDPEG
64ALDPEG - Safety Data Sheet
Safety Data Sheet (DEU) Aldosterone Kit / 64ALDPEH
64ALDPEH - Safety Data Sheet
Safety Data Sheet (ELL) Aldosterone Kit / 64ALDPEH
64ALDPEH - Safety Data Sheet
Safety Data Sheet (FRA-FR) Aldosterone Kit / 64ALDPEH
64ALDPEH - Safety Data Sheet
Safety Data Sheet (ITA) Aldosterone Kit / 64ALDPEH
64ALDPEH - Safety Data Sheet
Safety Data Sheet (SPA) Aldosterone Kit / 64ALDPEH
64ALDPEH - Safety Data Sheet
Safety Data Sheet (ENG-GB) Aldosterone Kit / 64ALDPEH
64ALDPEH - Safety Data Sheet
Safety Data Sheet (ENG-US) Aldosterone Kit / 64ALDPEH
64ALDPEH - Safety Data Sheet
Safety Data Sheet (DEU) Aldosterone Kit / 64ALDPEJ
64ALDPEJ - Safety Data Sheet
Safety Data Sheet (ELL) Aldosterone Kit / 64ALDPEJ
64ALDPEJ - Safety Data Sheet
Safety Data Sheet (FRA-FR) Aldosterone Kit / 64ALDPEJ
64ALDPEJ - Safety Data Sheet
Safety Data Sheet (ITA) Aldosterone Kit / 64ALDPEJ
64ALDPEJ - Safety Data Sheet
Safety Data Sheet (SPA) Aldosterone Kit / 64ALDPEJ
64ALDPEJ - Safety Data Sheet
Safety Data Sheet (ENG-GB) Aldosterone Kit / 64ALDPEJ
64ALDPEJ - Safety Data Sheet
Safety Data Sheet (ENG-US) Aldosterone Kit / 64ALDPEJ
64ALDPEJ - Safety Data Sheet
Plate Reader Requirement
Choosing the right microplate reader ensures you’ll get an optimal readout. Discover our high performance reader, or verify if your lab equipment is going to be compatible with this detection technology.
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