Acetylcholine Receptor Autoantibody ELISA kit ELISA
Contributes to the diagnosis of neuromuscular junction failure in myasthenia gravis (Erb-Goldflam disease)
Monitor myasthenia gravis
- ACCURATE AND RELIABLE ENZYMATIC ASSAY
The assay is based on competition between AChRAb from the human serum and MAb1 (coated on the microplate), and/or MAb2 and/or MAb3 (labeled with biotin) in binding to the Acetylcholine Receptor (AChR). The bound MAb2 and MAb3-biotin are detected by the addition of Streptavidin Peroxidase (SA-POD). The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate TMB.
Assay procedure: 16h at 2-8°C + 1 h at RT / shaking + 1 h at RT / shaking + 30 min at RT / shaking + 30 min at RT in the dark.
Working range: 0.5 – 20 nmol/L
Sample: serum – 100 µL
Analytical sensitivity: 0.25 nmol/L