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21-hydroxylase-autoantibody ELISA kit

21-hydroxylase-autoantibody ELISA kit ELISA

The 21-OH-AB kit is an accurate enzymatic assay measuring 21-hydroxylase autoantibodies in human serum.

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The 21-OH-AB kit is an accurate enzymatic assay measuring 21-hydroxylase autoantibodies in human serum.

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Overview

Identify patients with autoimmune Addison's disease

Screen patients with adrenal autoimmune diseases

Benefits

ACCURATE AND RELIABLE ENZYMATIC ASSAY

Assay principle: sandwich

21OH molecules are coated onto the ELISA plate wells. 21OH-biotin complexes constitute the liquid phase. 21OH-Ab bound to the 21OH immobilized onto the well will interact with 21OH-biotin to form a bridge. Addition of streptavidin peroxidase (SA-POD) detects the bound 21OH-biotin. The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate TMB. Assay procedure: 16-20h at 2-8°C + 1 h RT / shaking + 20 min RT / shaking + 20 min RT in the dark.