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21-hydroxylase-autoantibody ELISA kit ELISA

The 21-OH-AB kit is an accurate enzymatic assay measuring 21-hydroxylase autoantibodies in human serum.

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The 21-OH-AB kit is an accurate enzymatic assay measuring 21-hydroxylase autoantibodies in human serum.

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In stock

Overview

Identify patients with autoimmune Addison's disease

Screen patients with adrenal autoimmune diseases

Benefits

  • ACCURATE AND RELIABLE ENZYMATIC ASSAY

Assay principle: sandwich

21OH molecules are coated onto the ELISA plate wells. 21OH-biotin complexes constitute the liquid phase. 21OH-Ab bound to the 21OH immobilized onto the well will interact with 21OH-biotin to form a bridge. Addition of streptavidin peroxidase (SA-POD) detects the bound 21OH-biotin. The unbound SA-POD in excess is washed off before the addition of the peroxidase substrate TMB. Assay procedure: 16-20h at 2-8°C + 1 h RT / shaking + 20 min RT / shaking + 20 min RT in the dark.

Features

Working range: 0.3 – 100 U/mL

Sample: serum – 50 µL

Analytical sensitivity: 0.03 U/mL

Normal values

Cut-off 21-OHAb U/mL
Negative <0.4
Positive = 0.4

Product Insert (ENG) 21OH / 21E--96

21E/96 Op.Mod.001 - Product Insert

Product Insert (FRA) 21OH / 21E--96

21E/96 Op.Mod.001 - Product Insert

IVD Product list 2020

Immunoassays RIA and ELISA - Catalog

Catalogue produits IVD 2020

Immuno-analyse RIA et ELISA - Catalog

Annual availability Schedule 2020

In-vitro RIA products - Availability calendar

Calendrier de mise a disposition 2020

Trousse pour radioimmunologie - Availability calendar

Safety Data Sheet 21OH / 21E/96

21E/96 - Safety Data Sheet

Quality Control Report 21OH / 21E--96

21E/96 Batch K21E23D - Quality Control Report