17 alpha-hydroxyprogesterone radioimmunoassay kit RIA
Diagnosis congenital hyperplasia of the adrenal glands early
Monitor treatment for congenital hyperplasia of the adrenal glands
- LOW ANALYTICAL DETECTION LIMIT
The principle of the assay is based on competition between the 17a-OHP radiolabeled with iodine-125 and the 17a-OHP to be assayed contained in the samples for a given number of antibody binding sites adsorbed onto the tube walls. NB: extraction step for pregnant women at the 3rd trimester and newborn children.
One-step assay procedure: 2h30 at 37°C.
Working range: 0.09 – 75.7 nmol/L [0.10 – 25 ng/mL] (nmol 17 a-hydroxyprogesterone/L = ng/mL x 3.03)
Sample: serum, EDTA or heparin plasma – 25 µL
Analytical sensitivity: 0.09 nmol/L
|Population||17 OHP ng/mL||17OHP nmol/L|
|Reproductive aged women|
|Follicular phase||0.10 - 0.80||0.303 - 2.42|
|Luteal phase||0.27 - 2.90||0.82 - 8.78|
|Normal men||0.31 - 2.17||0.94 - 6.57|
|Third trimester pregnant women*||2 - 12||6.06 - 36.36|
|Newborn*||<0.7 - 2.5||2.12 - 7.5|