17 alpha-hydroxyprogesterone radioimmunoassay kit RIA

The OHP-CT kit is a one-step radioimmunoassay for 17 a-hydroxyprogesterone (17a-OHP) to diagnose and monitor congenital hyperplasia of the adrenal glands.

Download the protocols

The OHP-CT kit is a one-step radioimmunoassay for 17 a-hydroxyprogesterone (17a-OHP) to diagnose and monitor congenital hyperplasia of the adrenal glands.

Size

Cat. number selected :

Quantity

In stock

Overview

Diagnosis congenital hyperplasia of the adrenal glands early

Monitor treatment for congenital hyperplasia of the adrenal glands

Benefits

LOW ANALYTICAL DETECTION LIMIT

Assay principle: competition

The principle of the assay is based on competition between the 17a-OHP radiolabeled with iodine-125 and the 17a-OHP to be assayed contained in the samples for a given number of antibody binding sites adsorbed onto the tube walls. NB: extraction step for pregnant women at the 3rd trimester and newborn children.

One-step assay procedure: 2h30 at 37°C.

Features

Working range: 0.09 – 75.7 nmol/L [0.10 – 25 ng/mL] (nmol 17 a-hydroxyprogesterone/L = ng/mL x 3.03)

Sample: serum, EDTA or heparin plasma – 25 µL

Analytical sensitivity: 0.09 nmol/L

Normal values

Population	17 OHP ng/mL	17OHP nmol/L
Reproductive aged women
Follicular phase	0.10 - 0.80	0.303 - 2.42
Luteal phase	0.27 - 2.90	0.82 - 8.78
Post ACTH	<3.2	<9.69
Normal men	0.31 - 2.17	0.94 - 6.57
Third trimester pregnant women*	2 - 12	6.06 - 36.36
Newborn*	<0.7 - 2.5	2.12 - 7.5